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Construction of Piezo1-downregulated cell lines. (a) Piezo1 mRNA expression in different ESCC cell lines. (b) Protein expression of Piezo1 in transfected EC9706 and EC109 cell lines. (c and d) Relative mRNA expression of Piezo1 in transfected EC9706 and EC109 cell lines. * p < 0.05 vs control and *** p < 0.001 vs control.
