| Strains |
|
C. glutamicum CICC 21763 |
l-lysine-producing strain |
CICC |
|
C. glutamicum 0206 |
derived from CICC
21763 through mutagenesis |
laboratory stock |
|
E. coli DH5α |
plasmids holding strain |
laboratory stock |
|
E. coli MG1655 |
wild-type strain |
laboratory stock |
|
B. subtilis 168 |
wild-type strain |
laboratory stock |
|
S.
cerevisiae W303-1A |
wild-type strain |
laboratory stock |
| CgΔexeR |
C. glutamicum 0206 with ∼1 kb deletion of exeR
|
this study |
| Cg-Pst |
C. glutamicum 0206
with pXMJ19*ptxDPst
|
this study |
| Cg-Pae |
C. glutamicum 0206
with pXMJ19*ptxDPae
|
this study |
| Cg-Kpn |
C. glutamicum 0206
with pXMJ19*ptxDKpn
|
this study |
| CgΔexeR*Pst |
C. glutamicum 0206 with ∼1 kb deletion of exeR, Peftu, and ptxDPst inserted at this deletion region |
this study |
| Plasmids |
| pXMJ19 |
<keep-together>E. coli–C. glutamicum</keep-together> shuttle vector;
Cmr Ptac lacIq pMB1 oriVE. coli pBL1 oriVC. glutamicum
|
(26) |
| pXMJ19*ptxDPst
|
pXMJ19 containing Peftu and ptxDPst
|
this study |
| pXMJ19*ptxDPae
|
pXMJ19 containing Peftu and ptxDPae
|
this study |
| pXMJ19*ptxDKpn
|
pXMJ19 containing Peftu and ptxDKpn
|
this study |
| pJYS3_crtYf
|
pBL1tsoriVC. glutamicum Knr pSC101 oriVE. coli PlacM-FnCpf1, Pj23119-crRNA targeting crtYf, 1 kb upstream and downstream homologous arms flanking 705-bp deletion fragment inside crtYf
|
(26) |
| pJYS3ΔexeR
|
pBL1tsoriVC. glutamicum Knr pSC101 oriVE. coli PlacM-FnCpf1, Pj23119-crRNA targeting exeR, 1 kb upstream and downstream homologous arms flanking 1125-bp deletion fragment inside exeR
|
this study |
| pJYS3ΔexeR-Peftu-ptxDPst
|
derived from pJYS3_ΔexeR; Peftu (290 bp) and ptxDPst (∼1 kb) inserted between the 1 kb upstream and downstream homologous region flanking
the 1125-bp deletion fragment inside exeR
|
this study |
