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. 2021 May 12;12:674325. doi: 10.3389/fphar.2021.674325

TABLE 1.

Effects of anti-inflammatory therapeutics in OP models.

OP model Pretreatments Therapeutic class Therapeutic agent Dosing regimen Outcome References
Rat—Sarin (108 ug/kg, i.m.) None NSAID Indomethacin Ibuprofen 10 mg/kg, at onset of convulsions ↑ seizure severity Chapman et al. (2019)
Rat—Sarin (108 ug/kg, i.m.) None Steroid Methylprednisolone Dexamethasone 20 mg/kg, at onset of convulsions ↑ seizure severity Chapman et al. (2019)
Rat—Sarin (108 ug/kg, i.m.) None Steroid Methylprednisolone Dexamethasone 20 mg/kg ip at 4, 20 hr after sarin No improvement in clinical seizure severity or PGE2 levels at 24 and 48 h. Chapman et al. (2019)
Rat—Sarin (108ug/kg, i.m.) None COX-2 Inhibitor Nimesulide 6 mg/kg at 4, 20 h after sarin ↓ TNFa, PGE2, IL1B, and IL6 expression at 8 and 24 h; Chapman et al. (2019)
No improvement to neuropathology.
Rat—Sarin (108 ug/kg, i.m.) None COX inhibitor Ibuprofen 10 mg/kg at 4, 20 h after sarin ↓ IL6 expression at 24 h; Chapman et al. (2019)
No improvement to neuropathology.
Rat—Sarin (108 ug/kg, i.m.) None Phospholipase A2 Inhibitor Quinacrine 5 mg/kg at 4, 20 h after sarin ↓ IL1B and IL6 expression at 24 h; Chapman et al. (2019)
No improvement to neuropathology.
Rat—Sarin (108 ug/kg, i.m.) None PGE Analogue Ilomedin Prostin Misoprostol Immediately after sarin, again at 2 hr after sarin ↓ TNFa, IL1B, and IL6 expression at 24 h; Chapman et al. (2019)
Prostin and misoprostol ↓ TSPO expression at 24 h;
At 7 d, all-or-none reduction in TSPO.
Rat—Soman (154 ug/kg, s.c.) 30 m asoxime chloride (125 mg/kg, i.m.) Antioxidant AEOL10150 7 mg/kg, s.c. 1, 5, or 15 min after SE onset, repeated every 4 h ↓ oxidative stress, microglial activation, neurodegeneration, and proinflammatory cytokine expression at 24 h. Liang et al., (2019)
Rat—Soman (180 ug/kg, s.c.) 30 m asoxime chloride (125 mg/kg, i.m.) Immune modulator Poly-YE 1 mg/kg, s.c. 24 h post intoxication ↓ neurodegeneration and microglial activation in the piriform cortex at 28 d; Finkelstein et al. (2012)
No significant improvements in Barnes Maze performance.
Rat—DFP (9.5 mg/kg, i.p.) 30 m with pyridostigmine bromide (0.1 mg/kg, s.c.), 10 m with atropine methylbromide (20 mg/kg, s.c.) EP2 receptor antagonist TG6-10–1 5 mg/kg, i.p. Treatment iii) ↓ delayed mortality, ↑ weight gain, ↓ transcription of cytokines/chemokines, ↓ IBA1 mRNA and immunolabeling, ↓ FJB in CA1 at 4 d. Rojas et al. (2015)
i) 1 h prior to DFP;
ii) two injections (4 and 21 h after SE-onset);
iii) six injections (80–150 min, 5–6 h, 9–21 h, 31–42 h, and 48 hr after SE-onset)
Rat—DFP (9.5 mg/kg, i.p.) 30 m with pyridostigmine bromide (0.1 mg/kg, s.c.), 10 m with atropine methylbromide (20 mg/kg, s.c.) EP2 receptor antagonist TG6-10–1 5 mg/kg, i.p. Treatment iii) improved discrimination in the NOR at 4 w post intoxication. Rojas et al. (2016)
i) 1 h prior to DFP;
ii) three injections (1.5, 6, 21 h after SE-onset);
iii) six injections (1.5, 6, 21, 30, 45–47, and 52–55 h after SE-onset)
Rat—DFP (4 mg/kg, s.c.) None iNOS inhibitor 1400 W 20 mg/kg every 12 h for 3 d ↓ GFAP and IBA1 cells at 7 d; Putra et al. (2019)
↓ incidence, duration, and frequency of SRS over 12 w;
↓ reactive astrogliosis and microgliosis at 12 w.
Rat—DFP (4.5 mg/kg, s.c.) 30 m pyridostigmine (0.1 mg/kg, i.m.) Antioxidant AEOL10150 5 mg/kg s.c. 5 min into SE, repeated every 4 h ↓ proinflammatory mediators at 24 h. Liang et al. (2018)
Rat—DFP (5 mg/kg, i.p.) None Immune modulator Naltrexone 5 mg/kg starting 1 h post intoxication, repeated daily. Reduced learning deficits over 4 w post intoxication. Brewer et al. (2013)
Rat—DFP (9 mg/kg, i.p.) 30 m pyridostigmine bromide (0.1 mg/kg, i.m.), 10 m atropine methylnitrate (20 mg/kg, i.m.) Growth factor Neuregulin-1 (NRG-1) 3.2 ug/kg, internal carotid artery Treatment i) at 24 h ↓ neurodegeneration and oxidative stress; ↓ shifts in microglia activation; ↓ expression of proinflammatory cytokines; Li et al. (2012); Li et al. (2015)
i) 5 min pretreatment; Treatment ii) ↓ neurodegeneration at 24 h.
ii) post-treatment 1 hr after DFP intoxication;
iii) post-treatment 4 h after DFP intoxication
Rat—DFP (4 mg/kg, s.c.) None Antioxidant Diapocynin (DPO) 300 mg/kg, p.o., six doses, 12 h intervals beginning 2 h post-DFP Mitigates motor deficits at 18 d; Putra et al. (2020)
↓ astrogliosis, neurodegeneration, and inflammatory cytokine expression at 6 w.
Rat—Paraoxon (0.45 mg/kg, i.m.) None Immune modulator Poly-YE 1 mg/kg, s.c. 24 h post intoxication ↓ neurodegeneration in the piriform cortex and amygdala at 28 d; Finkelstein et al. (2012)
↓ microglial activation and ↑ BDNF in the piriform cortex at 28 d.

DFP, diisopropylfluorophosphate; TMB4, trimedoxime bromide; MDZ, midazolam; AMN, atropine methyl nitrate; AS, atropine sulfate; 2-PAM, pralidoxime; SE, status epilepticus; ↑, increase; ↓, decrease; FJB, Fluoro-Jade B; NOR, novel object recognition.