Fig. 2. Eosinophilia mediates enhanced bone metastasis in Il-5 Tg mice.

(A) The number of eosinophils in BM of WT and Il-5 Tg mice, n = 4 mice per group. (B) Comparison of metastasis foci of spines on day 14. WT and Il-5 Tg mice were injected with B16-F10 cells via caudal arteries. (C) The proportion of metastasis in the spine from mice injected with B16-F10 intravenously (WT, n = 20; Il-5 Tg, n = 26). (D) Representative photograph of metastatic foci in the spine of WT and Il-5 Tg mice injected with B16-F10 intravenously on day 14. (E) The number of the spine metastasis in (D). (F) Schematic representation of BM transplantation followed by establishing a metastasis model. (G) Frequencies of eosinophils in BM of WT mice that have received donor BM transplants from WT, Il-5 Tg, or Il-5 Tg and Eos-null mice 28 days ago. (H) Comparison of the metastatic foci in the spine on day 42. (I) Quantification of the tumor burden per area (mm²) in the spine on day 42. (B, E, and G to I) n = 6 to 8 mice per group. Statistical analyses were performed by Student’s t test (A, B, and E), one-way ANOVA (G, H, and I), and Fisher’s exact test (C). Data (mean ± SEM) are from one representative experiment of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.