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. 2021 May 26;7(22):eabg4302. doi: 10.1126/sciadv.abg4302

Fig. 5. Suppression of ISC synthesis increases IRP2-mRNA binding independent of altered IRP2 stability at tissue O2 levels.

Fig. 5

(A and B) EMSA using 32P-labeled IRE probe (A), or immunoblot for indicated proteins (B), of lysates derived from parental and IRP1 knockout HEK-293 cell line expressing dox-inducible ISCU-DN, treated with 100 nM dox (1 day), and incubated in 21% O2, lysis in 1% O2. (C and D) EMSA using 32P-labeled IRE probe (C) and immunoblot for indicated proteins (D) of lysates derived from parental and IRP1 knockout MDA-MB-231 cell line expressing shGFP or shNFS1, incubated in 3% O2, lysis in 1% O2.