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. 2021 May 26;7(22):eabg4302. doi: 10.1126/sciadv.abg4302

Fig. 7. IRP1/2 double-knockout cells expressing TFR1 can proliferate without iron supplementation and are sensitive to ferroptosis, but cannot sense ISC deficiency and enhance ferroptosis sensitivity.

Fig. 7

(A) Proliferation (relative cell number) of MDA-MB-231 cells expressing dox-repressible IRP2, TFR1 open reading frame (TFR1 without IRE) with 100 nM dox as indicated. (B) Immunoblots of IRP1/IRP2 double-knockout MDA-MB-231 cells expressing dox-repressible IRP2, TFR1 open reading frame, and nontargeting shRNA (shGFP, −) or shRNA targeting NFS1 (shNFS1, +), treated with 100 nM dox. (C) Relative viability of cells as in (B), treated with 100 nM dox, 1 μM ferrostatin, or erastin (0, 2.5, 5, and 10 μM) for 2 days as indicated. (D) Immunoblots of MDA-MB-231 cells expressing dox-repressible IRP2, TFR1 open reading frame, and shGFP or shRNAs targeting FXN (shFXN, 1 or 2), treated with 100 nM dox. (E) Relative viability of cells as in (D), treated with 100 nM dox, 50 μM Trolox, or erastin (0, 2.5, 5, and 10 μM) for 2 days as indicated. Asterisks indicate significance comparing shGFP to shNFS1 or shFXN for each group. P < 0.05, error bars are SEM.