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. Author manuscript; available in PMC: 2021 May 26.
Published in final edited form as: Bioconjug Chem. 2020 May 27;31(6):1624–1634. doi: 10.1021/acs.bioconjchem.0c00163

Figure 4.

Figure 4.

A) SDS-PAGE gel of the reactions of CdSrtA3M where the X position varied. Reactions (200 μM enzyme, 200 μM NSpaA, 5 mM DTT, and 1 mM Peptide) were measured after 24 hours. The majority of the more active sorting signals were non-polar while the polar residues tended to decrease the activity relative to the original sorting signal (LPLTG). B) SDS-PAGE gel of the reactions of CdSrtAΔ where the X position varied in the LPLTG Peptide. Reactions (200 μM enzyme, 200 μM NSpaA, 5 mM DTT, and 1 mM Peptide) were measured after 24 hours. C) Images were analyzed with Image J which estimated the amount of product produced for each peptide in the library.