Table 4. NLP Peptides Identified in the First-Stage (L1) and Third-Stage Larvae (L3) of H. contortus.
| NLP | precursor namea | database | sequence | modifications | mass | start positionb | end positionb |
|---|---|---|---|---|---|---|---|
| nlp-1c | HCOI00158100 | PRJEB506.WBPS10 | AVMFPRTFGALFG | Gly-loss+Amide | 1354.722 | 31 | 43 |
| nlp-1c | HCOI00158100 | PRJEB506.WBPS10 | MDMKHYFVGLG | Gly-loss+Amide | 1238.594 | 82 | 92 |
| nlp-3 | HCON_00188680 | PRJEB506 | AINPFLDSMG | Gly-loss+Amide | 1005.495 | 28 | 37 |
| nlp-3 | HCON_00188680 | PRJEB506 | AVNPFLDSFG | Gly-loss+Amide | 1007.508 | 40 | 49 |
| nlp-3 | HCON_00188680 | PRJEB506 | SSRYQPYYHLD | unmodified | 1427.647 | 52 | 62 |
| nlp-3 | HCON_00188680 | PRJEB506 | YFDSLAGQALG | Gly-loss+Amide | 1082.54 | 65 | 75 |
| nlp-5 | HCON_00046600; maker-C469189-snap-gene-0.14-mRNA-1 | PRJEB506; PRJNA205202 | ALSSFDTLGGIGLG | Gly-loss+Amide | 1248.671 | 42 | 55 |
| nlp-5 | HCON_00046600 | PRJEB506 | TQLSSIDSLGGLGLG | Gly-loss+Amide | 1358.741 | 46 | 60 |
| nlp-5 | HCON_00046600; maker-C469189-snap-gene-0.14-mRNA-1 | PRJEB506; PRJNA205202 | SEDTAKKALSSFDTLGGIGLG | Gly-loss+Amide | 2008.048 | 123 | 143 |
| nlp-5 | HCON_00046600; maker-C469189-snap-gene-0.14-mRNA-1 | PRJEB506; PRJNA205202 | DDMLAGEKKSVSSFDTLAGIGLG | Gly-loss+Amide | 2252.136 | 146 | 168 |
| nlp-5 | maker-C469189-snap-gene-0.14-mRNA-1 | PRJNA205202 | SRLFSTYYYLPYRDSLEDMDQNAQE | unmodified | 3103.387 | 171 | 195 |
| nlp-5 | HCON_00046600 | PRJEB506 | SRLFSTYYYLPYRDSLEDMDQNVQE | unmodified | 3131.418 | 127 | 151 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | LVIPSYLSSHYD | unmodified | 1392.693 | 32 | 43 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | TLDFDDPRLFSTAFG | Gly-loss+Amide | 1642.799 | 46 | 60 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | NGLVTTTLNRPRFI | unmodified | 1600.905 | 63 | 76 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | SPFLGTNVGLAYI | unmodified | 1350.718 | 79 | 91 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | ADMDPRFISNSFG | Gly-loss+Amide | 1397.64 | 94 | 106 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | STLYDFDDPRFASLSFG | Gly-loss+Amide | 1878.879 | 109 | 125 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | SGFDFDDPRFSSMSFG | Gly-loss+Amide | 1739.725 | 128 | 143 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | SGFDLEDPRFASMSFG | Gly-loss+Amide | 1703.761 | 146 | 161 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | SGFN FEDPRFASLSFG | Gly-loss+Amide | 1718.805 | 164 | 179 |
| nlp-7 | HCON_00165470; maker-C452207-snap-gene-0.3-mRNA-1 | PRJEB506; PRJNA205202 | SGFDLDDPRFASMSFG | Gly-loss+Amide | 1689.746 | 182 | 197 |
| nlp-7 | HCON_00165470 | PRJEB506 | SGSDLEDPRYWSMSFG | Gly-loss+Amide | 1774.762 | 200 | 215 |
| nlp-8 | HCON_00010710; maker-scaffold14524-snap-gene-0.4-mRNA-1 | PRJEB506; PRJNA205202 | AFDRIESN DFGLF | unmodified | 1529.715 | 49 | 61 |
| nlp-8 | HCON_00010710; maker-scaffold14524-snap-gene-0.4-mRNA-1 | PRJEB506; PRJNA205202 | AFDRIEMADFGF | unmodified | 1417.634 | 69 | 80 |
| nlp-8 | HCON_00010710; maker-scaffold14524-snap-gene-0.4-mRNA-1 | PRJEB506; PRJNA205202 | AFDRVGRTEFGFEGVL | unmodified | 1798.9 | 85 | 100 |
| nlp-8 | HCON_00010710; maker-scaffold14524-snap-gene-0.4-mRNA-1 | PRJEB506; PRJNA205202 | TEFGFEGVL | unmodified | 997.4757 | 92 | 100 |
| nlp-8 | HCON_00010710; maker-scaffold14524-snap-gene-0.4-mRNA-1 | PRJEB506; PRJNA205202 | AADRLADIGFRN | unmodified | 1317.679 | 104 | 115 |
| nlp-9 | HCON_00136940; maker-scaffold10126-snap-gene-0.11-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFHGYFNMPSS | unmodified | 1597.71 | 48 | 62 |
| nlp-9 | HCON_00136940; maker-scaffold10126-snap-gene-0.11-mRNA-1 | PRJEB506; PRJNA205202 | LSGEYPYYLYE | unmodified | 1395.623 | 65 | 75 |
| nlp-9 | maker-scaffold10126-snap-gene-0.11-mRNA-1 | PRJNA205202 | GGGRAFFGGWQPYESLGARMD | unmodified | 2258.033 | 78 | 98 |
| nlp-9 | HCON_00136940; maker-scaffold10126-snap-gene-0.11-mRNA-1 | PRJEB506; PRJNA205202 | SSSLWEFLEDRNAL | unmodified | 1665.8 | 127 | 140 |
| nlp-10 | HCON_00073270; maker-scaffold13486-augustus-gene-0.18-mRNA- | PRJEB506; PRJNA205202 | AVMPFSGGLYG | Gly-loss+Amide | 1039.516 | 64 | 74 |
| nlp-10 | HCON_00073270; maker-scaffold13486-augustus-gene-0.18-mRNA- | PRJEB506; PRJNA205202 | SEMPDDMYIERPVLPLSAGWQE | unmodified | 2562.177 | 90 | 111 |
| nlp-10 | HCON_00073270; maker-scaffold13486-augustus-gene-0.18-mRNA- | PRJEB506; PRJNA205202 | AVMPFSGGLYGKRAVMPFSGGLYG | Gly-loss+Amide | 2403.223 | 114 | 137 |
| nlp-10 | HCON_00073270; maker-scaffold13486-augustus-gene-0.18-mRNA- | PRJEB506; PRJNA205202 | AAMPFSGGLYG | Gly-loss+Amide | 1011.485 | 140 | 150 |
| nlp-10 | HCON_00073270; maker-scaffold13486-augustus-gene-0.18-mRNA- | PRJEB506; PRJNA205202 | ADRYIRSPMPISGGIFG | Gly-loss+Amide | 1777.93 | 153 | 169 |
| nlp-10 | HCON_00073270; maker-scaffold13486-augustus-gene-0.18-mRNA- | PRJEB506; PRJNA205202 | SPMPISGGIFG | Gly-loss+Amide | 1003.516 | 159 | 169 |
| nlp-11 | HCON_00062360; augustus-C472037-abinit-gene-0.0-mRNA-1 | PRJEB506; PRJNA205202 | LETELHPLVMGMYGFGPENNAY | unmodified | 2481.135 | 36 | 57 |
| nlp-11 | HCON_00062360; augustus-C472037-abinit-gene-0.0-mRNA-1 | PRJEB506; PRJNA205202 | HISPSFDVEEDVGNMRTLMDIG | Gly-loss+Amide | 2403.12 | 67 | 88 |
| nlp-11 | HCON_00062360; augustus-C472037-abinit-gene-0.0-mRNA-1 | PRJEB506; PRJNA205202 | QLSVADDVGRQMQMYHRLFEAG | Gly-loss+Amide | 2492.205 | 91 | 112 |
| nlp-11 | HCON_00062360; augustus-C472037-abinit-gene-0.0-mRNA-1 | PRJEB506; PRJNA205202 | AALSPSQDLQSAVELSNYLERAG | Gly-loss+Amide | 2360.197 | 116 | 138 |
| nlp-12 | HCON_00021180; maker-scaffold5805-snap-gene-0.2-mRNA-1 | PRJEB506; PRJNA205202 | DYRPLQFG | Gly-loss+Amide | 936.4818 | 31 | 38 |
| nlp-12 | HCON_00021180; maker-scaffold5805-snap-gene-0.2-mRNA-1 | PRJEB506; PRJNA205202 | DGYRPLQFG | Gly-loss+Amide | 993.5032 | 41 | 49 |
| nlp-12 | HCON_00021180; maker-scaffold5805-snap-gene-0.2-mRNA-1 | PRJEB506; PRJNA205202 | SPLASAFLVPAL | unmodified | 1184.681 | 62 | 73 |
| nlp-13 | HCON_00136950; maker-C471727-snap-gene-0.19-mRNA-1 | PRJEB506; PRJNA205202 | NDFSRDIMHFG | Gly-loss+Amide | 1279.577 | 33 | 43 |
| nlp-13 | HCON_00136950; maker-C471727-snap-gene-0.19-mRNA-1 | PRJEB506; PRJNA205202 | AYGNGRLVAYGGPAFERDMMAFG | Gly-loss+Amide | 2391.125 | 46 | 68 |
| nlp-13 | HCON_00136950; maker-C471727-snap-gene-0.19-mRNA-1 | PRJEB506; PRJNA205202 | SGGFEREMMSFG | Gly-loss+Amide | 1275.538 | 71 | 82 |
| nlp-13 | HCON_00136950 | PRJEB506 | SPFEREFLSFG | Gly-loss+Amide | 1256.61E | 85 | 95 |
| nlp-13 | HCON_00136950; maker-C471727-snap-gene-0.19-mRNA-1 | PRJEB506; PRJNA205202 | GSEFDREMLSFG | Gly-loss+Amide | 1315.587 | 98 | 109 |
| nlp-13 | HCON_00136950; maker-C471727-snap-gene-0.19-mRNA-1 | PRJEB506; PRJNA205202 | DEFERSMMAFG | Gly-loss+Amide | 1260.527 | 112 | 122 |
| nlp-14 | HCON_00190000; maker-scaffold4554-snap-gene-0.8-mRNA-1 | PRJEB506; PRJNA205202 | ALDSLEGDGFGGLF | unmodified | 1396.651 | 52 | 65 |
| nlp-14 | HCON_00190000; maker-scaffold4554-snap-gene-0.8-mRNA-1 | PRJEB506; PRJNA205202 | SLDSLEGDGFGFD | unmodified | 1357.567 | 68 | 80 |
| nlp-14 | HCON_00190000; maker-scaffold4554-snap-gene-0.8-mRNA-1 | PRJEB506; PRJNA205202 | ALNALDGTGFGFD | unmodified | 1296.599 | 83 | 95 |
| nlp-14 | HCON_00190000; maker-scaffold4554-snap-gene-0.8-mRNA-1 | PRJEB506; PRJNA205202 | ALNSLEGTGFGFD | unmodified | 1326.609 | 98 | 110 |
| nlp-14 | HCON_00190000; maker-scaffold4554-snap-gene-0.8-mRNA-1 | PRJEB506; PRJNA205202 | SLNSIEGTGFGFD | unmodified | 1342.604 | 113 | 125 |
| nlp-14 | HCON_00190000; maker-scaffold4554-snap-gene-0.8-mRNA-1 | PRJEB506; PRJNA205202 | SLDSTEGTGFGYRRG | Gly-loss+Amide | 1543.738 | 160 | 174 |
| nlp-14 | HCON_00190000; maker-scaffold4554-snap-gene-0.8-mRNA-1 | PRJEB506; PRJNA205202 | TLPQITGTHPYLRLY | unmodified | 1771.962 | 177 | 191 |
| nlp-15 | HCON_00024600; maker-C472057-snap-gene-0.5-mRNA-1 | PRJEB506; PRJNA205202 | AFDSLSGSGLTPFN | unmodified | 1411.662 | 47 | 60 |
| nlp-15 | HCON_00024600; maker-C472057-snap-gene-0.5-mRNA-1 | PRJEB506; PRJNA205202 | AFDSLAGSGFTGFD | unmodified | 1390.604 | 79 | 92 |
| nlp-15 | HCON_00024600 | PRJEB506 | SPIALNGGYYQPLREDALLL | unmodified | 2202.169 | 95 | 114 |
| nlp-17 | HCON_00114940 | PRJEB506 | NSLSN MMRLG | Gly-loss+Amide | 1063.527 | 44 | 49 |
| nlp-17 | HCON_00114940 | PRJEB506 | VDALKEQEPCVDCSLGNLMRLG | 2 Cys-Cys; Gly-loss+ | 2329.123 | 52 | 73 |
| nlp-18 | HCON_00035340; augustus-scaffold7830-abinit-gene-0.1-mRNA-1 | PRJEB506; PRJNA205202 | SDEVVEDDGELE | unmodified | 1334.536 | 52 | 63 |
| nlp-18 | HCON_00035340; augustus-scaffold7830-abinit-gene-0.1-mRNA-1 | PRJEB506; PRJNA205202 | SPYRQFAFA | unmodified | 1085.529 | 74 | 82 |
| nlp-18 | HCON_00035340 | PRJEB506 | GSPYGFAFA | unmodified | 915.4127 | 85 | 93 |
| nlp-19c | HCOI00354200 | PRJEB506.WBPS10 | IGMRLPNIIYM | unmodified | 1319.709 | 52 | 62 |
| nlp-20 | HCON_00131138 | PRJEB506 | DLDNSKKFSFA | unmodified | 1270.619 | 64 | 74 |
| nlp-20 | HCON_00131138 | PRJEB506 | FADRDDRLIR | unmodified | 1275.668 | 105 | 114 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGGRAFVPIEE | unmodified | 1130.572 | 27 | 37 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFFGDE | unmodified | 1025.457 | 39 | 48 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFVVDT | unmodified | 991.5087 | 51 | 60 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGGRAFLPVEE | unmodified | 1130.572 | 73 | 83 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFFKDD | unmodified | 1082.515 | 85 | 94 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFVPVKEEDEGQSFTDLE | unmodified | 2380.118 | 97 | 118 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFFVP | unmodified | 920.4868 | 121 | 129 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFFVPK | unmodified | 1048.582 | 121 | 130 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGGRAFLPIEE | unmodified | 1144.588 | 132 | 142 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFFQEP | unmodified | 1078.52 | 144 | 153 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGARAFP | unmodified | 674.35 | 156 | 162 |
| nlp-21 | HCON_00067330; maker-C471673-augustus-gene-0.17-mRNA-1 | PRJEB506; PRJNA205202 | GGGRAFVPQ | unmodified | 887.4614 | 165 | 173 |
| nlp-24 | HCON_00126360 | PRJEB506 | VNPLQGAMMGAMMGAMRG | Gly-loss+Amide | 1763.813 | 66 | 83 |
| nlp-31 | HCON_00105250 | PRJEB506 | PVVVERTIIYPG | Gly-loss+Amide | 1283.76 | 68 | 79 |
| nlp-35c | HCOI00356900; snap-scaffold16547-abinit-gene-0.6-mRNA-1 | PRJEB506.WBPS10; PRJNA205202 | DQLAFSGQNAYLRQLLQN LKPK | unmodified | 2544.381 | 46 | 67 |
| nlp-37 | HCON_00163850 | PRJEB506 | NNAEVVNHILKNFGTLDRLGDVG | Gly-loss+Amide | 2436.287 | 60 | 83 |
| nlp-40 | HCON_00017810.1; maker-C461709-augustus-gene-0.1-mRNA-1 | PRJEB506; PRJNA205202 | QLTAPTTMEKEEA | Gln->pyro-Glu | 1430.66 | 41 | 53 |
| nlp-40 | HCON_00017810.1; maker-C461709-augustus-gene-0.1-mRNA-1 | PRJEB506; PRJNA205202 | MVAWQPM | unmodified | 861.3877 | 67 | 73 |
| nlp-42 | HCON_00142640 | PRJEB506 | SAVGELSYPRRFL | unmodified | 1493.799 | 36 | 48 |
| nlp-42c | HCOI01162100 | PRJEB506.WBPS10 | SVDWHSLGWAWG | Gly-loss+Amide | 1341.626 | 57 | 68 |
| nlp-44 | HCON_00163905 | PRJEB506 | STLPLSSLLVPYPRVG | Gly-loss+Amide | 1639.966 | 34 | 49 |
| nlp-44 | HCON_00163905 | PRJEB506 | SFFTGDRNVYPPTSI | unmodified | 1699.821 | 52 | 66 |
| nlp-44 | HCON_00163905 | PRJEB506 | RYLYTARVG | Gly-loss+Amide | 1039.593 | 79 | 87 |
| nlp-54 | HCON_00096380 | PRJEB506 | GNM WGTPSKSYGYTN LA E | unmodified | 1974.878 | 43 | 60 |
| nlp-58 | HCON_00137190 | PRJEB506 | SLYGVDDGFTFKGFRGL | unmodified | 1877.931 | 41 | 57 |
| nlp-58 | HCON_00163905 | PRJEB506 | MPYMNLKGLRG | Gly-loss+Amide | 1220.652 | 62 | 72 |
| nlp-59 | HCON_00096380; maker-scaffold14296-augustus-gene-0.22-mRNA-1 | PRJEB506; PRJNA205202 | FEGLADYVALEDPNA | unmodified | 1622.746 | 63 | 77 |
| nlp-59 | HCON_00096380; maker-scaffold14296-augustus-gene-0.22-mRNA-1 | PRJEB506; PRJNA205202 | LAILSARGFG | Gly-loss+Amide | 945.576 | 85 | 94 |
| nlp-67 | HCON_00186320; maker-scaffold993-snap-gene-0.2-mRNA- | PRJEB506; PRJNA205202 | AVPVEVEQRE | unmodified | 1154.593 | 54 | 63 |
| nlp-67 | HCON_00186320; maker-scaffold993-snap-gene-0.2-mRNA- | PRJEB506; PRJNA205202 | SYPRNCYFSPIQCLFT | 2 Cys-Cys | 1935.865 | 71 | 86 |
| nlp-68 | HCON_00070810; augustus-scaffold17454-abinit-gene-0.0-mRNA-1 | PRJEB506; PRJNA205202 | LVREPPLI | unmodified | 935.5804 | 50 | 57 |
| nlp-68 | HCON_00070810; augustus-scaffold17454-abinit-gene-0.0-mRNA-1 | PRJEB506; PRJNA205202 | GIDPFSIPTLIKEPPL | unmodified | 1735.976 | 60 | 75 |
| nlp-68 | HCON_00070810; augustus-scaffold17454-abinit-gene-0.0-mRNA-1 | PRJEB506; PRJNA205202 | DSYVYPSTNSQLSDRIPLREPPL | unmodified | 2646.329 | 82 | 104 |
| nlp-69c | HCOI01768700 | PRJEB506.WBPS10 | FYRTGGTILLG | Gly-loss+Amide | 1138.65 | 58 | 68 |
| nlp-71 | HCON_00122860 | PRJEB506 | ALNQFKNCYFSPIQCVLME | 2 Cys-Cys | 2245.037 | 62 | 80 |
| nlp-74 | HCON_00082180; maker-scaffold4879-augustus-gene-0.4-mRNA-1 | PRJEB506; PRJNA20222 | APQMYDDVQFV | unmodified | 1311.581 | 28 | 38 |
| nlp-74 | HCON_00082180; maker-scaffold4879-augustus-gene-0.4-mRNA-1 | PRJEB506; PRJNA205202 | SNAELINGLIGMDLGKLSAVG | Gly-loss+Amide | 2013.093 | 41 | 61 |
| nlp-74 | HCON_00082180; maker-scaffold4879-augustus-gene-0.4-mRNA-1 | PRJEB506; PRJNA205202 | SNAELINGLLGMNLNKLSSAG | Gly-loss+Amide | 2057.094 | 64 | 84 |
| nlp-81 | HCON_00107730 | PRJEB506 | FTNGDFFVP | unmodified | 1101.524 | 48 | 56 |
| nlp-81 | HCON_00107730 | PRJEB506 | WIPSGGAGLVSGRG | unmodified | 1312.689 | 60 | 73 |
| nlp-81 | HCON_00107730 | PRJEB506 | DWRSAIAEPNF | unmodified | 1304.615 | 83 | 93 |
| HCON_00135420 | HCON_00135420; maker-scaffold6512-augustus-gene-0.7-mRNA-1 | PRJEB506; PRJNA205202 | ARNPYSWMVVDQS | unmodified | 1551.714 | 45 | 57 |
| HCON_00135420 | HCON_00135420; maker-scaffold6512-augustus-gene-0.7-mRNA-1 | PRJEB506 | SRNPYSWMVHSKG | Gly-loss+Amide | 1489.725 | 60 | 72 |
| HCON 00135420 | HCON_00135420; maker-scaffold6512-augustus-gene-0.7-mRNA-1 | PRJEB506; PRJNA205202 | ARNPYSWMNE | unmodified | 1266.545 | 101 | 110 |
a
The precursor name corresponds either to the one reported in project PRJEB506 or to the one reported in project PRJNA205202 on the WormBase site.
b
When sequences are predicted by both databases, the peptide sequence start and end positions refer to the database indicated first.
c
Denotes identified sequences from the PRJEB506 release (versions to 10).