Table 1. Summarized Binding Parameters for the Interaction of Flavonols (Kaempferol, Quercetin, and Myricetin) with CCL2 Orthologsa.
| name of protein | quenching constant (Kq) (m–1 s–1) | dissociation constant (Kd) (μM) | number of binding sites | R2 |
|---|---|---|---|---|
| Kaempferol (KP) | ||||
| mCCL2-WT | 5.0 × 1012 | 24 ± 6 | 1.1 ± 0.2 | 0.96 |
| hCCL2-WT | 5.2 × 1012 | 26 ± 5 | 1.1 ± 0.1 | 0.97 |
| mCCL2-M | 5.1 × 1012 | 26 ± 5 | 1.2 ± 0. 1 | 0.97 |
| hCCL2-M | 5.3 × 1012 | 25 ± 4 | 1.1 ± 0.1 | 0.98 |
| Quercetin (QT) | ||||
| mCCL2-WT | 4.3 × 1012 | 3.9 ± 0.5 | 1.2 ± 0.2 | 0.98 |
| hCCL2-WT | 4.2 × 1012 | 3.5 ± 0.7 | 1.1 ± 0.1 | 0.99 |
| mCCL2-M | 5.7 × 1012 | 3.7 ± 0.4 | 1.1 ± 0.2 | 0.98 |
| hCCL2-M | 5.4 × 1012 | 3.6 ± 0.5 | 1.2 ± 0.1 | 0.99 |
| Myricetin (MT) | ||||
| mCCL2-WT | 6.1 × 1012 | 0.42 ± 0.03 | 1.1 ± 0.2 | 0.99 |
| hCCL2-WT | 5.8 × 1012 | 0.38 ± 0.04 | 1.3 ± 0.2 | 0.98 |
| mCCL2-M | 6.3 × 1012 | 0.40 ± 0.03 | 1.2 ± 0.1 | 0.99 |
| hCCL2-M | 6.2 × 1012 | 0.43 ± 0.02 | 1.2 ± 0.2 | 0.98 |
a
M represents monomer and WT represents wild type. For all CCL2 proteins, the concentrations were obtained in terms of the monomer.