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. 2021 Jun;27(6):1616–1626. doi: 10.3201/eid2706.203096

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This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Phylodynamic analysis of region 1 in a curated study of echovirus 30 (E30) viral protein 1 (VP1) sequences from 22 countries in Europe, 2010–2018. We constructed the bootstrapped maximum likelihood neighbor-joining trees using 47 full length sequences and 277 VP1 sequences extracted from GenBank. E30 groups 1–8 are labeled. A) Maximum likelihood trees constructed by using MEGA version 7.0 (https://www.megasoftware.net). Prototype E30 strain Bastianni, (GenBank accession no. AF311938) was used as a reference. Scale bar indicates nucleotide substitutions per site. B) Maximum likelihood trees constructed by using Nextstrain (https://nextstrain.org) from which we dropped several problematic sequences, including group 5.