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. 2021 Apr 15;6(16):10975–10983. doi: 10.1021/acsomega.1c00798

Figure 3.

Figure 3

In vitro verifications of the predicted candidates. (a) Plasmid schematic of pET28a-ilvA, (b) SDS-PAGE of purified proteins, and (c) relative activities of TDs with 0–10 mM isoleucine. The absolute activities are 70.5 ± 0.2, 18.8 ± 0.1, 15.3 ± 0.1, 44.3 ± 0.1, 55.5 ± 0.1, 7.3 ± 0.1, and 60.1 ± 0.2 mM/(min·mg protein) for WT, G445M, F440T, G445K, P441L, S443A, and L360Y of TDs. All of the data have been tested in triplicates. The relative activities are calculated by the initial slope of the average data with R2 > 0.99.