Table 4. Effect of Acute, Subacute, and Teratogenicity on Hematological Parametersa.
| subacute
toxicity |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 5 mg/kg |
10 mg/kg |
20 mg/kg |
40 mg/kg |
||||||||
| hematology parameters | control | acute oral toxicity | terato-genicity | M | F | M | F | M | F | M | F |
| WBC’s | 3.7 ± 0.12 | 13.8 ± 0.1* | 12.8 ± 0.2* | 3.6 ± 0.42 | 6.8 ± 0.2* | 5.2 ± 0.2* | 5.2 ± 0.1* | 4.0 ± 0.3 | 8.0 ± 0.3* | 6.0 ± 0.1* | 8.8 ± 0.2* |
| RBC’s | 5.95 ± 0.32 | 6.67 ± 0.6 | 5.65 ± 1.1 | 6.12 ± 0.3 | 5.92 ± 1.1 | 6.13 ± 0.1 | 5.11 ± 1.2 | 6.94 ± 1.2 | 6.53 ± 2.1 | 5.89 ± 1.1 | 6.55 ± 1.3 |
| platelets | 817 ± 0.14 | 603 ± 0.52 | 800 ± 0.2 | 659 ± 0.4 | 750 ± 0.2 | 615 ± 0.21 | 750 ± 0.3 | 817 ± 0.12 | 888 ± 0.1 | 650 ± 0.32 | 806 ± 0.3 |
| Hb | 12.0 ± 1.2 | 12.8 ± 0.3 | 12.0 ± 0.14 | 12.9 ± 0.1 | 12.7 ± 0.1 | 13.0 ± 0.1 | 11.2 ± 0.1 | 12.9 ± 0.1 | 12.5 ± 0.1 | 12.6 ± 1.1 | 12.9 ± 0.1 |
| LYM (×103/μL) | 3.1 ± 1.4 | 7.0 ± 2.1* | 10.9 ± 1.1* | 2.9 ± 1.0 | 4.6 ± 1.0 | 3.0 ± 1.1 | 3.0 ± 1.1 | 5.8 ± 0.9 | 4.3 ± 1.0 | 4.8 ± 1.0 | 5.1 ± 1.1 |
| MID (×103/μL) | 0.4 ± 1.3 | 5.9 ± 1.3 | 1.5 ± 0.5 | 0.7 ± 0.62 | 0.8 ± 0.3 | 0.8 ± 0.7 | 0.8 ± 0.5 | 6.3 ± 0.5 | 1.1 ± 0.6 | 5.4 ± 0.6 | 1.1 ± 0.6 |
| GRA (×103/μL) | 0.1 ± 0.02 | 0.8 ± 0.1* | 0.4 ± 0.1 | 1.0 ± 0.1* | 0.4 ± 0.0 | 0.4 ± 0.12 | 0.4 ± 0.2 | 0.5 ± 0.13 | 0.6 ± 0.6 | 0.3 ± 0.1 | 0.6 ± 0.1 |
| MCH (pg) | 20.30 ± 0.1 | 19.1 ± 0.8 | 19.6 ± 0.2 | 21.0 ± 0.3 | 21.6 ± 0.1 | 21.2 ± 0.2 | 22 ± 0.2 | 20.1 ± 0.2 | 20.6 ± 0.2 | 18 ± 0.12 | 19.9 ± 0.2 |
| MCHC (g/dL) | 36.5 ± 0.96 | 35.8 ± 0.2 | 33.5 ± 0.8 | 35.5 ± 0.7 | 34.1 ± 0.8 | 36.2 ± 0.8 | 36.1 ± 0.8 | 34.9 ± 0.8 | 35.9 ± 0.9 | 34.4 ± 0.7 | 35.1 ± 0.7 |
| MCV (fl) | 55.4 ± 1.2 | 53.6 ± 1.0 | 58.6 ± 0.2 | 59.1 ± 0.2 | 53.3 ± 0.1 | 58.6 ± 0.1 | 51.0 ± 0.2 | 57.7 ± 0.1 | 57.5 ± 0.1 | 52 ± 0.2 | 56.7 ± 0.2 |
| HCT (%) | 33.0 ± 3.2 | 36.2 ± 2.1 | 33.1 ± 1.2 | 36.2 ± 1.3 | 37.5 ± 1.2 | 35.6 ± 1.5 | 31.2 ± 1.5 | 22.7 ± 1.1 | 37.5 ± 1.1 | 30.9 ± 2.4 | 37.1 ± 1.2 |
a
The data are represented as mean ± SEM with n = 5 for acute toxicity, n = 10 for teratogenicity, and n = 10 for subacute toxicity studies. *P < 0.05 was the increased level of significance compared to the control. M = male and F = female.