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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Toxicol Sci. 2020 Dec 1;178(2):281–301. doi: 10.1093/toxsci/kfaa147

Figure 1.

Figure 1.

Alginate Immobilization of Metabolic Enzymes (AIME) lid design. The AIME platform consists of a custom polystyrene lid with 96 solid supports functionalized for enhanced hydrogel attachment that is compatible with all ANSI/SLAS standard microplates (A). AIME lid with solid supports containing microspheres of hepatic S9 attached in an alginate matrix. The column on the left contains microspheres dyed with Ponceau-S to aid in visualization (B). Each solid support in a standard 96-well microplate provides sufficient distance between the end of the solid support and the well bottom for attachment of the microsphere without impacting the cell monolayer (C).