Figure 5.

AIME-coupled ERTA metabolism positive test set screening. The ΔAUC and confidence interval derived from ToxBoot uncertainty quantification analysis are plotted with mean ± quantile of 3. Significance of metabolic effects is noted for bioinactivation (blue), no metabolic effect (black), and bioactivation (red) (A). ToxCast pipeline (TCPL) curve fits for metabolism negative (control; blue) and metabolism positive (metabolism; red) assay modes. Vertical lines represent the potency (AC₅₀) for respective assay modes. Horizontal line is the mean response for test guideline E2 controls (grey solid line). Plots are shown for chemicals where significant metabolism-dependent effects were observed. Data points are normalized responses plotted for all experimental replicates (n=3) (B).