Table 1.

Summary Assay Performance Statistics for Screening Variability and Quality

		Metabolism
		Neg	Pos	Neg	Pos	Neg	Pos	Neg	Pos
NRS	Neg	0.90	NA	6.75	NA	2.77	NA	5.39	NA
	Pos	0.91	0.69	8.93	17.17	2.82	8.51	2.98	5.23
		Z’		CV: DMSO		CV: E2		CV: MXC

Inter-assay variability for negative (DMSO), positive (17β-estradiol; E2), and metabolism (methoxychlor; MXC) controls was determined by coefficient of variation (CV). ERTA screening quality denoted by Z′-factor (Z′) for negative and positive controls. Performance parameters were evaluated formetabolism negative (Neg) and positive (Pos) modes of the AIME assay ± supplementation of the cell culture medium with an NADPH regeneration system (NRS).

Abbreviation: NA, not applicable.