Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | K562 dCas9-KRAB |
Gilbert et al., 2014
doi:10.1016/j.cell.2014.09.029 |
||
| Cell line (Homo sapiens) | K562 control | This paper | K562 dCas9-KRAB transduced with pControlsgRNA | |
| Cell line (Homo sapiens) | K562 OCIAD1 sgRNA#2 | This paper | K562 dCas9-KRAB transduced with pOCIAD1sgRNA2 | |
| Cell line (Homo sapiens) | K562 OCIAD1 rescue | This paper | K562 OCIAD1 sgRNA#2 transduced with pUltra-OCIAD1 | |
| Cell line (Homo sapiens) | K562 OCIAD1 (F102A) rescue | This paper | K562 OCIAD1 sgRNA#2 transduced with pUltra-OCIAD1(F102A) | |
| Cell line (Homo sapiens) | U2OS dCas9-KRAB | This paper | U2OS transduced with pMH0006 (Addgene, cat# 135448; Chen et al., 2019) | |
| Cell line (Homo sapiens) | U2OS control | This paper | U2OS dCas9-KRAB transduced with pControlsgRNA | |
| Cell line (Homo sapiens) | U2OS OCIAD1 sgRNA #2 | This paper | U2OS dCas9-KRAB transduced with pOCIAD1sgRNA2 | |
| Cell line (Homo sapiens) | U2OS OCIAD1 rescue | This paper | U2OS OCIAD1 sgRNA#2 transduced with pUltra-OCIAD1 | |
| Cell line (Homo sapiens) | U2OS scramble | This paper | U2OS control cells transduced with pLKO.1-blast-Scramble (Addgene, cat# 26701) | |
| Cell line (Homo sapiens) | U2OS OCIAD2 shRNA#1 | This paper | U2OS control cells transduced with pLKO1-OCIAD2_shRNA1 | |
| Cell line (Homo sapiens) | U2OS double knockdown | This paper | U2OS OCIAD1 sgRNA#2 transduced with pLKO1-OCIAD2_shRNA1 | |
| Antibody | rabbit polyclonal anti-OCIAD1 | Invitrogen | PA5-20834 | RRID:AB_11155625
(1:2000-1:5000) |
| Antibody | mouse monoclonal anti-OCIAD1 | Proteintech | 66698–1-Ig | RRID:AB_2882051
(1:5000) |
| Antibody | Rabbit polyclonal anti-OCIAD2 | Invitrogen | PA5-59375 | RRID:AB_2644946
(1:500-1:5000) |
| Antibody | Mouse monoclonal anti-ATP5A1 | Proteintech | 66037–1-Ig | RRID:AB_11044196
(1:2000-1:5000) |
| Antibody | Rabbit polyclonal anti-NDUFB8 | Proteintech | 14794–1-AP | RRID:AB_2150970
(1:2000) |
| Antibody | Mouse monoclonal anti-SDHA | SantaCruz Biotechnology | sc-166947 | RRID:AB_10610526
(1:2000-1:5000) |
| Antibody | Rabbit polyclonal anti-UQCRC2 | Proteintech | 14742–1-AP | RRID:AB_2241442
(1:2000-1:5000) |
| Antibody | Mouse monoclonal anti-UQCRC1 | Invitrogen | 459140 | RRID:AB_2532227
(1:2000) |
| Antibody | Rabbit polyclonal anti-CYC1 | Proteintech | 10242–1-AP | RRID:AB_2090144
(1:1000) |
| Antibody | Mouse monoclonal anti-COXIV | Proteintech | 66110–1-1g | RRID:AB_2881509
(1:2000) |
| Antibody | Mouse monoclonal anti-PHB2 | Proteintech | 66424–1-Ig | RRID:AB_2811041
(1:5000) |
| Antibody | Rabbit polyclonal anti-TIM50 | Proteintech | 22229–1-AP | RRID:AB_2879039
(1:1000) |
| Antibody | Rabbit polyclonal anti-TOM70 | Proteintech | 14528–1-AP | RRID:AB_2303727
(1:1000) |
| Antibody | Mouse monoclonal anti-GFP | Proteintech | 66002–1-Ig | RRID:AB_11182611
(1:2000) |
| Antibody | Mouse monoclonal anti-β-actin | Proteintech | 66009–1-1g | RRID:AB_2687938
(1:10000) |
| Antibody | Rabbit polyclonal anti-GPD2 | Proteintech | 17219–1-AP | RRID:AB_2112476
(1:1000) |
| Antibody | Rabbit polyclonal anti-AIF | Proteintech | 17984–1-AP | RRID:AB_2224539
(1:1000) |
| Commercial assay or kit | SuperSignal West Femto | Thermo Scientific | 34094 | |
| Chemical compound, drug | Digitonin | Calbiochem | 300410 | |
| Chemical compound, drug | Lauryl maltose neopentyl glycol (LMNG) | Anatrace | NG310 | |
| Chemical compound, drug | Dithiobis(succinimidyl propionate) (DSP) | Life Technologies | 22585 | |
| Chemical compound, drug | LysC/Trypsin | Promega | V5071 | |
| Chemical compound, drug | ProteaseMAX | Promega | V2071 | |
| Other | μMACS protein A beads | Miltenyi Biotec | 130-071-001 | |
| Other | μ Columns | Miltenyi Biotec | 130-042-701 | |
| Other | μMACS Separator | Miltenyi Biotec | 130-042-602 | |
| Other | ZipTip with 0.6 µL C18 resin | Millipore Sigma | ZTC18S096 | |
| Other | Disposable gel cutter grids | The Gel Company | MEE2-7-25 | |
| Software, algorithm | DIA-NN | Version 1.7.12 and 1.7.13 (beta 1) | https://github.com/vdemichev/DiaNN | |
| Software, algorithm | ScreenProcessing | https://github.com/mhorlbeck/ScreenProcessing |