Figure 4.

Transgenic experiments determine the effect of OsGH3-2 on seed storability. A, Schematic gene model of OsGH3-2 showing the partial sequence alignment of two independent homozygous mutants (MT1 and MT2) and wild-type (WT) at the target site. The nucleotide differences among WT and MT and the protospacer adjacent motif (AGG) are marked. B, Germination percentage of the seeds of WT and CRISPR-induced OsGH3-2 mutants (MT) under AA conditions (88% RH, 43°C, 8 d). C, Relative transcript levels of the OX lines overexpressing OsGH3-2 in the developing seeds at 6 d after pollination. D, Germination percentage of the seeds of the WT and OX lines under AA conditions. E, Relative transcript levels of OsGH3-2 relative to UBIQUITIN in developing seeds of the WT and OsGH3-2-RNAi lines at 6 d after pollination. F, Germination percentage of the seeds of the WT and RNAi lines under AA conditions. The data are provided with the means ± standard error (n = 3). Asterisks indicate significant differences compared with WT at P < 0.01 by Student’s t test