Skip to main content
. 2021 Feb 23;186(1):519–533. doi: 10.1093/plphys/kiab084

Figure 5.

Figure 5

Functional analysis of OsmiR408 in rice. A, Overall morphology of the 35S::miR408 transgenic and WT plants. Bar was 10 cm. B, miRNA northern blot analysis of OsmiR408 in the 35S::miR408 and WT plants. C, Leaf angle of the 35S::miR408 and WT plants. Bar was 1 cm. D, Sequence deletions in homozygous miR408KO-1 and miR408KO-2 plants. Red bases represent the mature OsmiR408 sequences, whereas red dashes indicate the deletions in the 408KO-1 and 408KO-2 lines. E, miRNA northern blot analysis of OsmiR408 transcripts in the miR408KO-1 and miR408KO-2 plants. F, Grain morphology of the WT, miR408KO-1, and miR408KO-2 lines. G, Statistical analysis of the grain length and width of the WT and the 408KO lines (n = 3). H, Statistical analysis of the KGW of the WT and 408KO lines (n = 3). Comparisons were made compared to the respective WT unless otherwise indicated. Error bars represent ± sd, single and double asterisks in the statistical analyses represent significant difference determined by the Student’s t test at *P < 0.05 and **P < 0.01.