Figure 7.

Simplified scheme for reversible Cys OxiPTMs profiling approaches. Four main steps are outlined (orange boxes). After protein extraction, free –SHs are blocked and –SOXs (red bold font) are reduced indiscriminately (gray box) or specifically. Newly generated –SHs are then labeled, enriched, and quantified by various approaches. Plant studies are organized in a table, distinguishing studies analyzing –SOX (DTT/TCEP reduction, gray box) and –SNO (ascorbate reduction, white box). iodoTMTRAQ, combined iodoTMT and iTRAQ labeling; –SNO, S-nitrosothiol; –SOH, sulfenic acid; –SOX, reversible Cys OxiPTM; –SS–, disulfide.