Table 1.

Comparison of current techniques for Cys OxiPTM profiling

OxiPTM approaches	Advantages	Disadvantages
Chemoproteomics	Enable in situ labeling Direct and specific PTM recognition	Relative low availability and high cost of chemical probe Quantification thus far described only for two samples
Reductomics	High availability of labeling agents Multiplexed and absolute quantitation	Indirect in vitro workflow, risk of spontaneous oxidation Selectivity on specific reduction is questioned
Protein disulfide traps	In situ trapping within relevant redox context Enable subcellular organelle-specific trapping	Time-consuming preparation of transgenic materials In situ trapping influenced by endogenous redoxins No quantification