Figure 2.

Copper deficiency increases transcript abundance of BdYSL3. (A) The expression level of BdYSL3 in different tissues at different growth stages. Wild-type brachypodium was grown hydroponically under copper sufficient (0.25 µM CuSO₄) conditions. The indicated plant tissues were collected at the indicated time and developmental stage; RNA was extracted and subjected to RT-qPCR analysis. (B) The expression level of BdYSL3 in different tissues of brachypodium wild-type grown hydroponically under copper sufficient (+Cu) or deficient conditions (−Cu) conditions. Young (two uppermost leaves) and mature leaves (the remaining leaves), stems, and roots were collected from four-week-old plants. Flag leaves and flowers were collected from six-week-old plants. In all cases, the copper deficiency was achieved by transferring plants to a fresh medium lacking copper one week before tissue sampling. (C) Plants were grown hydroponically for two weeks before transferring to a medium without iron. Tissues were collected from three-week-old plants for subsequent RNA extraction, cDNA synthesis, and RT-qPCR. Rt, roots; ML, mature leaves; YL, young leaves. Shown values are arithmetic means ± S.E. (n = 3 independent experiments with tissues pooled from three plants per experiment. Levels not connected by same letter are significantly different (p < 0.05, Tukey-Kramer HSD test).