Figure 4.

BdYSL3 localizes to the plasma membrane in Arabidopsis thaliana protoplast. BdYSL3 fused with EGFP at the C-termini (A) or EGFP-expressing empty vector (B) was transfected into protoplasts prepared from A. thaliana mesophyll cells. Shown are a bright-field image (Bf) of transfected protoplasts, BdYSL3-EGFP (YSL3)-mediated fluorescence, EGFP (EGFP)-mediated fluorescence and chlorophyll autofluorescence (Chl) fluorescence. Overlay images were created to show that BdYSL3-EGFP-mediated fluorescence does not co-localize with the chlorophyll-mediated fluorescence. Scale bar = 5 µm.