Figure 8.

Copper delivery to flag leaves and flowers is impaired in the ysl3 mutant. ICP-MS-based analysis of the concentration of copper in roots (A), mature (two bottommost) leaves (B), flag leaves (C), and flowers (D) of wild-type plants (Wt), the ysl3-3 mutant (ysl3) and the ysl3-3 mutant expressing the YSL3 cDNA (YSL3-1). In (A) and (B), plants were grown hydroponically for four weeks with 250 nM CuSO₄ prior to tissue collection. In (C) and (D), tissues were collected from soil grown plants fertilized bi-weekly with N–P–K. (A–D) show mean values ± S.E. (n = 3 independent experiments). Levels not connected by the same letter are significantly different (p < 0.05, Tukey–Kramer HSD test). DW, dry weigh.