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. 2021 Feb 9;186(1):655–676. doi: 10.1093/plphys/kiab054

Figure 9.

Figure 9

The distribution of copper is altered in the ysl3-3 mutant. SXRF-based analysis of the spatial distribution of copper in mature leaves (A), flag leaves (B), and florets (C) of the indicated plant lines. Middle part of the leaf was used for SXRF imaging in both (A) and (B). White arrows in (C) point to anthers (An), stigma (S), and ovaries (Ov). Scale bar = 2 mm. Mature leaves in (A) were collected from three-week-old plants, grown hydroponically with 0.25 µM CuSO4. Flag leaves and florets were collected from soil-grown plants that were fertilized bi-weekly with N–P–K. (D) Two-dimensional confocal SXRF (2D-CSXRF) was used to visualize the spatial distribution of copper in node I of indicated plant lines. (E) illustrates the anatomy of the upper part of the node I. Part of the node in a rectangle was scanned using C-SXRF and is shown in D. Ph, phloem; Sh, leaf sheath; Vb, vascular bundle; Wt, wild type; X, xylem.