Table 1.

The ysl3-3 mutant has altered flower morphology and fertility.

Genotype	Flag leaf length (cm)	Spikeletsa	Floretsb	Seedsc	Fertile Florets (%)
Wt	7.6 ± 0.23	3.8 ± 0.22	39.8 ± 3.09	29.0 ± 6.71	76.2 ± 1.35
ysl3-3	4.9 ± 0.38*	7.7 ± 0.25*	70.0 ± 4.56*	31.0 ± 0.58	42.6 ± 3.33*
ysl3/YSL3-1	6.4 ± 0.44	4.8 ± 0.48	41.0 ± 2.52	31.3 ± 2.81	75.9 ± 3.76
ysl3 + 25 µM CuSO4	6.7 ± 0.56	5.0 ± 8.36	46.3 ± 8.36	29.0 ± 3.49	64.8 ± 4.92

Plants were grown in soil and fertilized bi-weekly with a standard N–P–K fertilizer. The ysl3-3 mutant was also grown in soil and in addition to N–P–K was also fertilized bi-weekly with 25 µM CuSO₄ (ysl3 + 25 µM CuSO₄). Spikes were collected at the end of the reproductive stage. We note that because the ysl3-3 mutant is developmentally delayed, its spikes were harvested and fertility was analyzed separately although all plant lines were sown and grown concurrently. Mean values ± SE are shown (n = 15 plants per each genotype). Asterisks (*) indicate statistically significant differences from the wild-type (p < 0.0001).

^aSpikelets include the terminal spikelet and lateral spikelets.

^bFlorets number indicates the total florets produced per spike.

^cSeeds number indicates the total seeds produced per spike.

^dFertile florets number was calculated as % of seeds formed per the number of florets per spike.