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. 2021 Jan 13;186(1):142–167. doi: 10.1093/plphys/kiaa117

Figure 2.

Figure 2

Molecular characterization of pgdh3 T-DNA insertion lines confirms diminished PGDH amounts and enzyme activity in leaves. A, Genomic locus map of the PGDH3 locus. Both T-DNA insertions are located in the first exon. Exons = dark gray boxes. B, Genotyping by PCR confirmation of homozygous insertions and gene disruption in pgdh3-1 and pgdh3-2. C, Above, immunoblotting of total protein extracted from WT and pgdh3 mutant plant leaf tissue using α-PGDH3. The WT 100, pgdh3-1, and pgdh3-2 lanes contain the equivalent of 2.5 mg fresh mass of leaf tissue. WT 20–80 lanes contain the corresponding percentage of the WT sample. Below, Ponceau red stain of the region of the blot occupied by the large subunit of rubisco (RbcL). D, Bar graph showing a drop of 46.2% in total leaf PGDH activity in pgdh3-1 (54% total PGDH protein content) and 38.3% in pgdh3-2 (60% total PGDH protein content) relative to WT (±sd, n ≥ 3 per genotype).