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. 2021 Feb 12;186(1):434–451. doi: 10.1093/plphys/kiab053

Figure 4.

Figure 4

TCP proteins disrupt the function of the MBW complex in repressing trichome formation on cotyledons. A, Firefly luciferase complementation assay showed that TCP4 interfered with the interaction between GL1 and GL3 in Arabidopsis protoplasts. The combinations are listed at the upper right corner. The combinations including GAL4BD-nluc and cluc-GL1, GL3-nluc and cluc-GAL4BD, and GAL4BD-nluc and cluc-GAL4BD were used as negative controls. The data are the means (±se) of three biological replicates. B, The transient expression assay showed that the activation of GL2 expression by GL1/GL3 was repressed by TCP4. The schematic diagram shows the constructs used in the transient expression assays. The GL2pro-LUC reporter was cotransformed with corresponding constructs. The data are the means (±se) of four biological replicates. The asterisks represent Student’s t test significance compared with GL1+GL3. ***P <0.001. C, The relative expression levels of GL2 in the 3- to 8-d-old cotyledons of wild-type and tcp2/3/4/5/10/13/17 plants. The expression level of GL2 in 3-d-old wild-type cotyledons was set as one. ACTIN8 was used as the reference gene. The data are the means (±se) of three biological replicates. The asterisks represent Student’s t test significance. **P < 0.01; ***P < 0.001. D–F, The genetic complementation of ectopic trichome on cotyledons of tcp2/3/4/5/10/13/17 mutants by loss-of-function of GL1 or GL3. Cotyledons of 6-d-old gl1 tcp2/3/4/5/10/13/17 (E) and gl3 tcp2/3/4/5/10/13/17 (F) mutants were glabrous. White arrows indicate the trichomes on tcp2/3/4/510/13/17 cotyledons (D). Bars = 500 μm.