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. 2021 Feb 23;186(1):239–249. doi: 10.1093/plphys/kiab083

Figure 1.

Figure 1

Expression levels of classical molecular markers of photomorphogenesis under specific de-etiolation conditions. (A) Representative image of seedlings grown for 2 d in the dark and then treated for 24 h with dark (D), WL or white light and fluridone (WL + F; left), as well as quantification of hypocotyl elongation (center) and cotyledon aperture (right) in seedlings subjected to the L or L + F treatments. Bar, 2.5 mm. Measurements from the initial and final time points are shown. Thick lines and shaded areas represent respectively the median and the interquartile range of at least 45 seedlings. (B) RT-qPCR analysis of the expression of the classical photomorphogenic marker genes LHCB1.4, CAB2, and LHCB2.2 in seedlings grown as in A. The expression of each gene was normalized to that of PP2A and transcript levels expressed relative to the value of darkness, set to one. Data represent mean ± SE of three biological replicates. Different letters denote statistically significant differences between means (Tukey test; P < 0.05).