Figure 3.

Conditioned media exhibited important bactericidal effects against Gram-positive bacteria. Approximately 1 × 10⁶ bacterial cells were exposed to CM from rat gut bacteria and incubated at 37 °C for 2 h. After this incubation, cultures were serially diluted, plated on nutrient agar, and incubated at 37 °C for 24 h. Bacterial colonies were enumerated on the following day. Data are expressed as the mean ± standard error of several independent experiments performed in duplicate. P values were determined using two sample t-tests. An asterisk (*) denotes P ≤ 0.05 (a) when CM were tested against B. cereus, (b) against S. pyogenes, and (c) against MRSA and (d) demonstrative effects of CM against MRSA and S. pyogenes.