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Comparative DNA complexation ability: (A) monotailed Neo–triazine conjugates 1_ST, 1_OL, and 1_Chol; (B) symmetric two-tailed Neo–triazine conjugates 2_ST, 2_OL, and 2_Chol; (C) asymmetric two-tailed Neo–triazine conjugates 1_ST-1_OL, 1_ST-1_Chol, and 1_OL1_Chol. Complexation was evaluated by monitoring the fluorochrome (SYBR Green I) exclusion from lipoplexes as a function of the charge ratio (CR, +/−).
