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. 2021 Jan 20;32(4):690–701. doi: 10.1021/acs.bioconjchem.0c00616

Figure 3.

Figure 3

Comparative cytotoxicity and transfection efficiency, evaluated on L929 cells, of complexes prepared by mixing pGL3 with Neo–triazine derivatives at different charge ratios (CR, +/−), 25 kDa bPEI and Lipofectamine 2000 (Lipo). Transfection efficiency is expressed as luminescence signal (RLU) normalized to the total protein content in each cell lysate: (A) monotailed Neo–triazine conjugates 1_ST, 1_OL, and 1_Chol; (B) symmetric two-tailed Neo–triazine conjugates 2_ST, 2_OL, and 2_Chol; (C) asymmetric two-tailed Neo–triazine conjugates 1_ST-1_OL, 1_ST-1_Chol, and 1_OL-1_Chol. Results are expressed as mean ± SD (n ≥ 3) (*, p < 0.05 vs other lipids for any given CR).