Table 1. Characterization Data of Polymers and Conjugates Synthesized in This Work.
| conjugates |
polymers |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % activation |
||||||||||
| sample | purity/%a | name | Mn(NMR)/kDab | no. of unitsb | yield/% | Đc | TNBSd | NMRb | log Pe | |
| 1 | My-PEG (10 kDa) | 64 | PEG | 10 | 166 | 61 | 1.1 | 73.4 | 90 | –0.3 |
| 2 | My-PMeEP (10 kDa) | 98 | PMeEP | 9.6 | 76 | 99 | 1.3 | 58.3 | 100 | 0.1 |
| 3 | My-PEtEP (10 kDa) | 91 | PEtEP | 10.5 | 75 | 99 | 1.2 | 57 | >100 | 1.2 |
| 4 | My-PEEP (10 kDa) | 98 | PEEP | 11.3 | 64 | 88 | 1.1 | >100 | 1.9 | |
| 5 | My-P(Et-Bu)EP (10 kDa) | 95 | P(Et-Bu)EP | 10.4 | 37–31 | 94 | 1.3 | 79 | 2 | |
| 6 | My-PEG (20 kDa) | 60 | PEG | 20 | 333 | 86 | 1.3 | 60.2 | 98 | –0.3 |
| 7 | My-PEtEP (20 kDa) | 83 | PEtEP | 21.6 | 157 | 96 | 1.1 | 57.5 | >100 | 1.2 |
a
Calculated by GPC in water with triple detection. The residual fraction was the unconjugated polymer that could not be removed during the Sephadex separation.
b
Calculated by 1H NMR.
c
Molar mass dispersity, defined as Đ = Mw/Mn.
d
Calculated by the TNBS assay. RDS is below 4%. Errors were evaluated on at least three replicas.
e
Partition coefficient calculated by the software ChemDraw on a dimeric unit of the polymer chain.