Table 3. Experimental and Best-Fit Parameters Obtained by Analyzing Native Myoglobin and Conjugates by Nano-DSC.
| experimental
curve |
best fit parameters
2-state model |
||||
|---|---|---|---|---|---|
| sample | T50/°Ca | Rev. %b | Δ°H/kJ mol–1 c | T50/°Ca | |
| My | myoglobin | 79.9 | 26 | 552 | 80.2 |
| 1 | My-PEG (10 kDa) | 75.5 | 81 | 391 | 75.8 |
| 2 | My-PMeEP (10 kDa) | 74.5 | 83 | 320 | 74.5 |
| 3 | My-PEtEP (10 kDa) | 72.9 | 74 | 299 | 73.0 |
| 4 | My-PEEP (10 kDa) | 62.7 | 15 | ||
| 5 | My-P(Et-Bu)EP (10 kDa) | 58.2 | 37 | ||
| 6 | My-PEG (20 kDa) | 76.6 | 73 | 409 | 76.7 |
| 7 | My-PEtEP (20 kDa) | 60.0 | 63 | ||
a
Data reported with an error bar of ±0.3 °C.
b
Data reported with an error bar of ±2 percentage points.
c
Data reported within an RSD of 5%.