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Salt-dependence of brush formation for 5 μM BRT–ES40. Frequency shift Δf vs time t. (a) Adsorption of BRT–ES40 from PB followed by washing with PBS (150 mM NaCl), followed by washing with a high salt buffer (PBS + 0.5 M NaCl). (b) Adsorption from PB followed by washing with PB (blue), compared with adsorption from PBS followed by washing with PBS (red).
