Figure 2.

Cofactors and bioconjugation assay. Two metathesis cofactors—N8 and Mes8—based on HG-II derived catalysts were synthesized (A). Binding analysis at varying ratios of HT to cofactor: (white with black outline) 50 μM HT: 50 μM cofactor; (blue) 50 μM HT: 110 μM cofactor; and (yellow with dots) 40 μM HT: 160 μM cofactor. All of the reactions were conducted in 20 mM MOPS, pH 7.0 at room temperature for 2 h (B).