Skip to main content
. 2021 May 12;11(10):6343–6347. doi: 10.1021/acscatal.1c01470

Figure 3.

Figure 3

Activity of the cofactors and corresponding ArMs. A reaction that produces the fluorescent 7-hydroxycoumarin was selected for facile characterization (A). The TON for the cofactors N8 and Mes8 were determined by GC-MS, UPLC-MS, and fluorescence analysis (B). The bioconjugation reactions were conducted at 65 μM cofactor and 55 μM HT (Supporting Information, section 11). The metathesis reactions were conducted in 20 mM MOPS, 100 mM MgCl2, pH 7.0 at 25 °C. Each reaction contained 2 μM cofactor, 2 μM HT, and 100 μM substrate. Formation of 7-hydroxycomarin was determined by fluorescence (λex = 330 nm and λem = 450 nm) and UPLC-MS; formation of naphthalene was determined by GC-MS (Figure S3).