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(A) Immunostaining of Tuj1 of MSCs grown on wrinkle gradients for (a) 1 week and (c) 2 weeks. (b, d) Zoomed picture of the typical position and quantitative results of Tuj1. Reprinted with permission from ref (173). Copyright 2020 Wiley-VCH. (B) (a) Immunostaining of ALP for cells cultured on roughness gradient after 4 and 21 days. (b) Quantitative results of ALP. Reprinted with permission from ref (181). Copyright 2014 Elsevier, Ltd.
