Features
of cell-based topographical microarrays. (A) Fluorescent
images ranking surfaces by nucleus roundness of human MSCs growing
on patterned PLA surfaces; the three lowest and three highest-ranked
surfaces are represented. The topographies are represented and colored
according to a Mann–Whitney U-test ranking. Cells show nuclear
(red) and cytoskeleton (green) staining. Reprinted with permission
from ref (144). Copyright
2015 Elsevier, Ltd. (B) High fidelity of PDMS patterns generated from
the MARC master molds by soft lithography. SEM images of (i) unpatterned
PDMS control, and (ii–viii) patterned topographies, with the
description of patterned size (line width or pillar and well diameter,
μm), pattern spacing (μm) and pattern height (μm),
in that order. The perpendicular pattern is also indicated with the
abbreviation (pr). Scale bar = 5 μm. Reprinted with permission
from ref (571). Copyright
2013 Elsevier, Ltd.