Figure 29.

Macrophage cell attachment is mediated by small circular pillar topography. (A) Macrophage cell attachment represented as high (blue), medium (green), or low (orange) plotted against total pattern area (μm²). Categories of macrophage attachment were determined through cluster analysis using Euclidian distance. Composite confocal images showing cell membrane (green) and nucleus (blue), representative of (B) low attachment, or (C) high attachment TopoUnits with inset (D) with orthogonal views of Z-stack images showing cellular engulfment of the cylindrical pillar feature. Scale bar = 10 μm. Reprinted with permission from ref (602). Copyright 2020 Wiley-VCH.