Figure 7.

CV staining of biofilm mutants in response to Sal4 SIgA. STm wild type strain AR05 and biofilm mutant strains ΔbcsA and ΔbcsE (cellulose), ΔwcaA (colanic acid), ΔyihO (O-Antigen capsule), and ΔcsgD (biofilm regulator) were grown to mid log phase and incubated in borosilicate glass tubes with 50 μg/mL Sal4 SIgA or control SIgA for 1 h at 37 °C under shaking conditions. Tubes were washed in PBS, fixed with methanol, and stained with 0.1% crystal violet (CV). (A) Representative images depict CV staining following antibody treatment. (B) Solubilized CV was quantified at A₅₅₀. Data represents three biological replicates each performed with three technical replicates. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc multiple comparison test. *p < 0.05.