Table 1. Agglutination of STm Cells by Sal4 mAbs by Flow Cytometry.

	% agglutinationa (mean ± SD)b
	Sal4 conc.c (μg/mL)
	2	20	200
IgG	5.82 ± 4.50	19.19 ± 11.18	33.51 ± 14.81
mIgA	11.05 ± 3.39	31.46 ± 4.99	79.04 ± 2.46
dIgA	12.41 ± 5.20	38.85 ± 10.01	91.12 ± 3.07
SIgA	10.30 ± 2.78	23.39 ± 7.85	66.38 ± 4.98

^aGating was set on untreated AR05 cells, and agglutination was defined by SSC-positive FSC-positive cells (Q2 + Q4). STm cells treated with isotype control antibodies resulted in Q2 + Q4 values <1%.

^bResults represent data from three separate biological experiments.

^cMid log phase AR05 cultures were washed in PBS and incubated with indicated amounts of Sal4 mAbs for 1 h at 37 °C. 10 000 events per sample were analyzed on a BD FACSCalibur (BD Biosciences, San Jose, CA) by forward scatter (FSC) and side scatter (SSC) to visualize aggregate size and granularity.