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Measurement of the activity of 31 DHB compounds against H. polygyrus L3 stage worms. Larval death is measured as the proportion of worms that respond to stimulus. Compounds were tested in duplicate at 100 μM. Dashed lines indicates the cutoff (50%) used to determine hits in this assay and the cutoff for good activity (75%):20 <50%, not active; 50–75%, moderate activity; >75%, good; and >90%, excellent activity.
