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. 2021 Apr 2;143(14):5413–5424. doi: 10.1021/jacs.1c00014

Figure 3.

Figure 3

Incorporation of tCO into full-length mRNA by T7 RNA polymerase assisted cell-free transcription. Denaturing agarose bleach gels showing RNA transcripts formed at five different tCOTP/CTP ratios (0–100%). Direct visualization of tCO fluorescence (a) and after ethidium bromide staining (b). RNA samples were heat-denatured (65 °C for 5 min, 1.5% bleach in the gel) prior to loading. (c) Same RNA transcripts upon harsher denaturation (70 °C for 10 min, 2% bleach in the gel). The RiboRuler High Range RNA ladder was used.