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RSL-sclx8 cocrystallization in the absence of precipitant at 4 °C. (A) pH dependence of crystal growth at 1 mM RSL, 10 mM sclx8 in 20 mM phosphate buffer, and 50 mM NaCl. Cocrystallization was triggered at pH ≤ 4.2. Microcrystalline precipitate occurred at pH 3.4 (Figure S5). (B) Cocrystallization dependence on the sclx8 concentration at pH 4.0. Cocrystallization was triggered at sclx8 ≥ 2.5 mM. Microscope images were acquired after 3 h incubation. Scale bars are 200 μm.
