Figure 5.

(A) Regions from overlaid ¹H–¹⁵N HSQC spectra of RSL during pH (gray scale) or sclx₈ (color scale) titrations. (B) Chemical shift perturbation plots of RSL backbone amides in response to pH adjustment (pH 4.0–3.0) or to the addition of 5 mM sclx₈ (at pH 4.0). Dashed lines indicate unassigned resonances due to overlap. (C) Detail of the RSL-sclx₈P3 assembly (Figure 2) showing RSL trimers in surface representation and bridging sclx₈ as black spheres or sticks. Significant chemical shift perturbations at 5 mM sclx₈ are highlighted blue.