Figure 4.
Normalized UV–vis spectra of (A) Fe–PPIX, (B) Fe–L1, and (C) Fe–L2 in E. coli lysates with (green) and without 16 h incubation with NADPH (blue). Biliverdin is highlighted in pink. Concentration = 50 μM, NADPH = 1 mM, 310 K.
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