Table 1. Results of Inhibition in the Stx1B ELISA Assaya.
| entry | Construct | ligand | valency (% functionalization of polymer) | IC50 (μM) | rel.pot.b | rel. pot. per sugarc |
|---|---|---|---|---|---|---|
| 1 | 1e | galabiose | 1 | 4968 ± 1232 | 4968 | 1 |
| 2 | 3 | galabiose | 2 | 1070 ± 283 | 4.6 | 2.3 |
| 3 | 4 | galabiose | 2 | 1245 ± 169 | 4 | 2 |
| 4 | 5 | galabiose | 4 | 19.9 ± 2.4 | 250 | 62.5 |
| 5 | 6 | galabiose | 4 | 13.5 ± 2.6 | 367 | 92 |
| 6 | 8 | globotriose | 10 (8%) | 2.8 ± 0.2 | 1,774 | 187 |
| 7 | 9 | galabiose | 20 (16%) | 0.0083 ± 0.0006 | 598,554 | 29,928 |
a
Determined in an ELISA-like assay with Stx1B (0.1 μg/mL) and wells coated with Gb3.
b
Relative to the potency of galabiose for 3, 4, 5, 6, and 9.
c
Relative potency divided by the valency.