Table 1. Phenolic Compounds in the Regular and Low Alcoholic Craft Beers Analyzed by HPLC-ESI-MS/MS.
| peak | name | tR (min) | [M – H–]− (m/z) | [M-174-H]− (m/z) | [M-162-H]− (m/z) |
|---|---|---|---|---|---|
| 1 | unknown | 2.87 | 301 | ||
| 2 | 3,4,5-trihydroxybenzoic acid (GA)a | 3.04 | 169 | ||
| 3 | caffeic acid-O-hexoside Ib | 3.37 | 179 | ||
| 4 | hydroxy-dimethoxybenzoic acid Ib | 3.78 | 197 | ||
| 5 | hydroxy-methoxybenzoic acid Ib | 4.02 | 167 | ||
| 6 | unknown | 4.12 | 285 | ||
| 7 | sinapic acid-O-hexoside Ib | 4.18 | 223 | ||
| 8 | 3,5-dihydroxybenzoic acidb | 4.21 | 153 | ||
| 9 | 3-caffeoylquinic acidb | 4.56 | 353 | ||
| 10 | ferulic acid-O-hexosideb | 4.70 | 193 | ||
| 11 | 3,4-dihydroxybenzoic acid (PCA)a | 5.00 | 153 | ||
| 12 | hydroxy-methoxybenzoic acid IIb | 5.34 | 167 | ||
| 13 | coumaric acid-O-hexosideb | 5.53 | 163 | ||
| 14 | sinapic acid-O-hexoside IIb | 5.96 | 223 | ||
| 15 | 2,5-dihydroxybenzoic acid (gentisic acid)b | 6.32 | 153 | ||
| 16 | coumaroylquinic acidb | 6.45 | 163 | ||
| 17 | sinapic acid-O-hexoside IIIb | 6.62 | 223 | ||
| 18 | 5-caffeoylquinic acid (CQA)a | 6.64 | 353 | ||
| 19 | hydroxy-dimethoxybenzoic acid IIb | 6.88 | 197 | ||
| 20 | trihydroxybenzoic acid Ib | 7.19 | 169 | ||
| 21 | 2,6-dihydroxybenzoic acidb | 7.24 | 153 | ||
| 22 | hydroxy-methoxybenzoic acid IIIb | 7.25 | 167 | ||
| 23 | unknown | 7.26 | 137 | ||
| 24 | 4-caffeoylquinic acidb | 7.33 | 353 | ||
| 25 | 3-feruloylquinic acidb | 7.47 | 193 | ||
| 26 | 4-hydroxybenzoic acid (pHBA)a | 7.72 | 137 | ||
| 27 | quercetin hexoside Ib | 8.74 | 301 | ||
| 28 | 4-hydroxy-3-methoxybenzoic acid (VA)a | 9.03 | 167 | ||
| 29 | hydroxy-dimethoxybenzoic acid IIIb | 9.12 | 197 | ||
| 30 | caffeic acid (CA)a | 9.38 | 179 | ||
| 31 | 4-hydroxy-3,5-dimethoxybenzoic acid (SyA)a | 9.39 | 197 | ||
| 32 | quercetin hexoside IIb | 9.53 | 301 | ||
| 33 | unknown | 9.61 | 609 | ||
| 34 | hydroxy-dimethoxybenzoic acid IVb | 10.71 | 197 | ||
| 35 | kampferol-3-O-hexosideb | 11.31 | 285 | ||
| 36 | 4-feruloylquinic acidb | 11.62 | 193 | ||
| 37 | trihydroxybenzoic acid IIb | 13.46 | 169 | ||
| 38 | p-coumaric acid (CuA)a | 14.04 | 163 | ||
| 39 | unknown | 14.41 | 223 | ||
| 40 | unknown | 14.89 | 609 | ||
| 41 | unknown | 15.22 | 609 | ||
| 42 | sinapic acid (SA)a | 15.86 | 223 | ||
| 43 | ferulic acid (FA)a | 16.05 | 193 | ||
| 44 | quercetin-3-O-rutinoside (Ru)a | 16.67 | 609 | ||
| 45 | kampferol-3-O-rutinosideb | 17.78 | [M-308-H]− = 285 | ||
| 46 | unknown | 17.78 | 301 | ||
| 47 | 2-hydroxybenzoic acid (salycilic acid)b | 19.39 | 137 | ||
| 48 | quercetin (Q)a | 27.68 | 301 | ||
| 49 | kampferol (K)a | 30.25 | 285 | ||
| 50 | isoxanthumolb | 32.76 | 353 |
a
Identified by comparison with standard.
b
Tentatively identified.