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. 2021 May 4;40(21):3719–3733. doi: 10.1038/s41388-021-01801-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Schematic representation of terminal α2,6-sialylation (α2,6NeuAc) of N-glycosylated proteins via ST6Gal1; B Positive Spearman’s rank correlation between the ERBB2 and ST6GAL1 transcript expression in gastric cancer (GC) patients from the DErrico dataset available at the Oncomine^TM database; C Western blot analysis of ErbB2 and ST6Gal1 expression in intestinal-type GC cell lines; D Immunofluorescence detection of ErbB2, ST6Gal1, and total (Sambucus nigra agglutinin (SNA)) and O-linked (sialyl Tn (STn)) α2,6-linked sialic acid (α2,6NeuAc) in intestinal-type GC cell lines; DAPI nuclear staining is shown in blue; The scale bar corresponds to 60 μm; E Western blot analysis of ErbB2 and α2,6NeuAc following receptor immunoprecipitation from NCI-N87 whole cell lysates and Peptide N-Glycosidase F (PNGase F) digestion; F Immunofluorescence detection of ST6Gal1 and its glycan product (α2,6NeuAc) in NCI-N87 WT and ST6GAL1 K.O. cells; DAPI nuclear staining is shown in blue; The scale bar corresponds to 60 and 40 μm, in the ST6Gal1 and SNA panels, respectively; G Western blot analysis of ST6Gal1 and α2,6NeuAc (SNA) expression in NCI-N87 WT and ST6GAL1 K.O. cells; C1 clone 1, C2 clone 2, C3 clone 3.