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. 2021 May 4;40(21):3719–3733. doi: 10.1038/s41388-021-01801-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Cell surface flow cytometric staining of NCI-N87 WT and ST6GAL1 K.O. ErbB2-positive cells with the (A) Sambucus nigra agglutinin (SNA) recognizing α2,6-linked sialic acid (α2,6NeuAc); B Maackia amurensis lectin II (MAL-II) recognizing α2,3-linked sialic acid (α2,3NeuAc); C CA 19.9 monoclonal antibody (mAb) recognizing sialyl Lewis a (SLe^a); D Aleuria aurantia lectin (AAL) recognizing α1,6- and α1,3/4-linked fucose (α1,6Fuc and α1,3/4Fuc); E Lotus tetragonolobus lectin (LTL) recognizing α1,2-linked fucose (α1,2Fuc); F Ulex europaeus agglutinin I (UEA-I) recognizing α1,2-linked fucose (α1,2Fuc); Comparisons were made using one-way ANOVA analysis of variance (n = 3; mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001); n.s. nonsignificant, C1 clone 1, C2 clone 2, C3 clone 3.